A Method of Preparing Recombinant Fusion Antigen from Rotavirus and Norovirus

• 영문명: A Method of Preparing Recombinant Fusion Antigen from Rotavirus and Norovirus
• 발행기관: 한국축산식품학회
• 저자명: Oh, Ho-Kyung Huh, Chul-Sung Baek, Young-Jin Yoo, Dong-Wan
• 간행물 정보: 『학술대회논문집』2004년도 정기총회 및 제33차 춘계 학술대회, 353~356쪽, 전체 4쪽
• 주제분류: 농수해양 > 식품과학
• 파일형태: PDF
• 발행일자: 2004.05.31

영문 초록

Rotavirus and Norovirus are major causative agents of acute diarrhea and gastroenteritis. In our study, Each viral RNA was isolated from the feces of patients for viral diarrhea in Korea, respectively. And cDNA library were constructed using RT-PCR. Also, cDNAs encoding VP8 derived from Rotavirus and Capsid protein derived from norovirus were subesequently cloned and expressed in Echerichia coli as a fusion antigen. Molecular weight of fusion antigen was approximately 60kDa. Also, substantial overexpression was accomplished. We yielded egg yolk lgY which is potentially useful in controlling of Rotavirus and Norovirus which are one of the most prevalent pathogenic viruses.

목차

Introduction

Materials and Methods

Results and Discussion

Summary