Effects of Hydrostatic Pressure on Myofibrillar Protein Extracted from Bovine Semitendinosus

• 영문명: Effects of Hydrostatic Pressure on Myofibrillar Protein Extracted from Bovine Semitendinosus
• 발행기관: 한국축산식품학회
• 저자명: Lee, Eun-Jung Kim, Yun-Ji Lee, Nam-Hyouck Yamamoto, Katsuhiro
• 간행물 정보: 『학술대회논문집』2004년도 정기총회 및 제33차 춘계 학술대회, 198~201쪽, 전체 4쪽
• 주제분류: 농수해양 > 식품과학
• 파일형태: PDF
• 발행일자: 2004.05.31

영문 초록

To investigate hydrostatic pressure (HP) effect on myofibrillar protein (Mf) extracted from bovine Semitendinosus muscle, Ca- and Mg-ATPase activities to evaluate denaturation of myosin and actin, and soluble protein contents were observed. In Mf treated with 100 MPa for 5 min was not observed denaturation of myosin and actin. In Mf treated with 200 MPa for 5 min, denaturation of myosin and actin were observed but inactivation rate was low (0.0136 min^-1 ). Inactivation rate of myosin and actin was dramatically increased above 300 MPa treatment. However denaturation of myosin and actin was not that critical with duration time. By increasing pressure size, the amount of myosin and actin in soluble protein eluted in 20 mM potassium phosphate buffer (pH 7.0) containing 0.6 M NaCl were decreased. SDS-PAGE of soluble protein released from Mf suspension in 0.1 M NaCl buffer (pH 7.0) showed that low molecular weight proteins (15~ 36 KDa) were released by HP treatment above 200 MPa. From the results, denaturation of myosin and actin, and release of light molecule proteins of Mf were observed by HP treatment over 200 MPa.

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Introduction

Materials and Methods

Results and Discussion

Summary