전라남도산림자원연구소 추출물은행 데이터 구축을 위한 난대수종의 추출물 분석 연구

• 영문명: Warm Temperate species extract Analysis for Jeollanamdo Forest Resources Institute extracts Bank Data building
• 발행기관: 한국자원식물학회
• 저자명: 김현우(Kim, Hyun-Woo) 조건웅(Jo, Geon-Ung) 여혜정(Yeo, Hye-jeong) 김현석(Kim, Hyeon-Seok) 어지현(Eo, JI-Hyun) 백효은(Beak, Hyo-Eun) 박종석(Park, Jong-Seok) 오찬진(Oh, Chan-Jin) 오득실(Oh, Deuk-sil) 박화식(Park, Hwa-Sik)
• 간행물 정보: 『한국자원식물학회 학술심포지엄』2019년도 춘계학술대회, 101~101쪽, 전체 1쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2019.04.30

영문 초록

This study developed a HPLC analysis method for the determination of avicularin (AL) and quercitrin (QT) in Astilbe chinensis by HPLC. HPLC was performed on a Capcell Pak C18 MGII column ($4.6{\times}250mm$, $5{\mu}m$) with a gradient elution of 0.05% (v/v) trifluoroacetic acid (TFA) and acetonitrile at a flow rate of 1.0 mL/min at $30^{\circ}C$ and a UV detection wavelength of 254 nm. The analytical method was validated for its specificity, precision, accuracy, and linearity. The limits of detection and quantitation were AL 0.09 and 0.28 mg/mL, QT 0.03 and 0.09 mg/mL respectively. Calibration curves showed good linearity (AL r2 > 0.99990 and QT r2 > 0.99994), and the precision of analysis was satisfied (less than AL 0.59%, QT 0.63%). Recoveries of quantified compounds ranged from AL 100.57 to 102.08%, QT 99.94 to 100.64%. These results indicate that the developed HPLC method was applied successfully to the detection of AL and QT in A. chinensis.

키워드

Astilbe chinensis Avicularin Functional cosmetic HPLC Quercitrin Validation