작은방울유리화법을 이용한 딸기 생장점 초저온동결보존

• 영문명: A successful regeneration from shoot tips of Fragaria x ananassa DUCH following cryopreservation by droplet-vitrification
• 발행기관: 한국자원식물학회
• 저자명: 이영이(Lee, Young-yi) 백형진(Baek, Hyung-Jin) 윤문섭(Yoon, Mun-Seop) 코트날라 발라라주(Balaraju, Kotnala) 송재영(Song, Jae-young)
• 간행물 정보: 『한국자원식물학회 학술심포지엄』2019년도 춘계학술대회, 80~80쪽, 전체 1쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2019.04.30

영문 초록

Ginseng seeds are one of short-lived seeds species which loose their viability easily in the condition of conventional storage. Cryopreservation using liquid nitrogen (LN) has been recommended as a alternative storage for this kind of germplasm short lived or dessiccation-sensitive. This study was performed to find out whether cryopreservation could affect physiological change such as enzyme activity induced by reactive oxygen species. In this work, the redox ratio of ascorbate and glutathione were examined onto ginseng seedlings before and after LN storage of seeds for 1 day using spectrophotometer method. Reduced ascorbate (ASA) was increased while oxidized ascorbate (DHA) was decreased slightly for both after 1d-LN storage. And for glutathione also, reduced form (GSH) was increased while oxidized form (GSSG) was decreased slightly for both after 1d-LN storage. Consequently total phenol compound and ion leakage after LN storage showed no significant differences. Additionally root growth from the seeds after LN storage was not affected by ultra low temperature. From the above results, we may suggest that cryopreservation could be recommended for storage tool of ginseng seeds even with low viability also and expected to make slower seed aging process during preservation period through further study.