멸종위기 흰복주머니란 종자발아에 미치는 요인 분석

• 영문명: Analysis of factors on the asymbiotic germination of white lady's slipper orchid(Cypripedium macranthos Sw. albiflorum)
• 발행기관: 한국자원식물학회
• 저자명: 이정관(Lee, Joung Kwan) 권영희(Kwon, Young Hee) 김희규(Kim, Hee Kyu) 김경옥(Kim, Kyung Ok) 박재성(Park, Jae Seong) 정미진(Jeong, Mi Jin) 손성원(Son, Sung Won) 서강욱(Suh, Gang Uk)
• 간행물 정보: 『한국자원식물학회 학술심포지엄』2019년도 춘계학술대회, 53~53쪽, 전체 1쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2019.04.30

영문 초록

Apple (Malus domestica) is one of the most economically important fruits in Korea. But virus infection has decreased sustainable production of apple and caused the serious problems such as yield loss and poor fruit quality. Virus or viroid infection including Apple chlorotic leaf spot virus (ACLSV), Apple stem pitting virus (ASPV), Apple stem grooving virus (ASGV), Apple mosaic virus (ApMV) and Apple scar skin viroid (ASSVd) has been also reported in Korea. In many cases, apple is infected with virus and viroid with no specific symptoms, the damage caused by the virus are unaware significantly. In our research, we tried to eliminate viruses in the rootstock for the disease-free seedlings of the apple dwarfing rootstock M.9 and M.26. The method of virus elimination was meristem culture, heat($37^{\circ}C$, 6weeks) treatment and chemistry($Ribavirin^{(R)}$) treatment. The analytical methods commonly used for the detection of virus is Enzyme-linked Immuno-Sorbent Assay(ELlSA) and Reverse Transcription-polymerase Chain Reaction(RT-PCR). RT-PCR method was more 30% sensitive than ELISA method. Efficiency of method eliminate virus appeared meristem method > heat treatment > chemistry treatment. The higher acquisition rate of disease-free seedlings is 30~40% on meristem treatment. In meristem treatment, the apple dwarfing rootstock M.9 gained infection ratio of ACLSV, ASPV and ASGV were 45%, 60% and 50% respectively. In the apple dwarfing rootstock M.26, infection ratio of ACLSV, ASPV and ASGV were 40%, 55%, 55%, respectively. Based on our results, it was found that most effective method of disease-free seedlings apple dwarfing rootstocks was by meristem treatment than heat method and chemistry treatment.

키워드

Apple disease-free seedlings ELlSA RT-PCR