사과 왜성대목 무독묘 생산을 위한 바이러스 제거 방법 비교

• 영문명: Comparison of Virus Elimination Methods for Disease-free Seedlings of the Apple Dwarfing Rootstock
• 발행기관: 한국자원식물학회
• 저자명: 권영희(Kwon, Young Hee) 이정관(Lee, Joung Kwan) 김희규(Kim, Hee Kyu) 김경옥(Kim, Kyung Ok) 박재성(Park, Jae Seong) 허윤선(Huh, Yoon Sun) 윤여중(Yoon, Yeo Joong)
• 간행물 정보: 『한국자원식물학회 학술심포지엄』2019년도 춘계학술대회, 54~54쪽, 전체 1쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2019.04.30

영문 초록

Strawberry which is the genus Fragaria under family Rosaceae is one of the most important fruit plants for both fresh consumption and food processing in the temperate and subtropical countries. Propagation of strawberry is achieved either through runners or by in vitro micropropagation. Meristem tips, generally obtained from runners of virus-free plants, are commonly used to establish in vitro cultures, which are employed for mass propagation or as a source of plant material for regeneration and transformation experiments. This study was conducted to determine the optimal natural additives strength to improve sprouting shoot rate of apical meristem of strawberry 'Seolhyang'. Strawberry apical meristem at size (0.2 mm to 0.3 mm) with leaf primordials were cultured on the 1/3MS(Murashige & Skoog) medium supplemented with five natural additives such as coconut milk, maple sap, banana powder and peptone. The sprouting ratio and growth characteristics were evaluated after eight weeks after in vitro culture. Shoot ratio of 'Seolhyang' apical meristem was 72.9% in 1/3MS medium supplemented with maple sap. On the other hand, the low shoot ratio was observed 47.7% in 1/3MS medium supplemented with banana powder. Shoot length was different as natural additives but numbers of leaf was not significantiy different among the natural additives. As a result, the sprouting ratio and plant growth were enhanced effectively in 1/3MS medium with maple sap compared to the others.

키워드

Strawberry Apical meristem Natural Additives