COX-2 inhibitory effect of luteolin through the regulation of NR-κB and ERK in phorbol ester activated human lung epithelial cells

• 영문명: COX-2 inhibitory effect of luteolin through the regulation of NR-κB and ERK in phorbol ester activated human lung epithelial cells
• 발행기관: 인문사회과학기술융합학회
• 저자명: Chung Mu Park
• 간행물 정보: 『예술인문사회융합멀티미디어논문지』7권 5호, 467~474쪽, 전체 8쪽
• 주제분류: 사회과학 > 사회과학일반
• 파일형태: PDF
• 발행일자: 2017.05.31

영문 초록

The inhibitory effect of luteolin on cyclooxygenase (COX)-2 expression was investigated in 12-O-tetradecanoyl phorbol-13-acetate (TPA), a prototype tumor promoter, activated human lung epithelial A549 cells. Luteolin mitigated TPA-stimulated COX-2 expression in a dose-dependent manner without any cytotoxicity. Since nuclear factor (NF)-κB plays a critical role in inflammatory casdades, activated status of NF-κB was estimated through the phosphorylation of p65, one subunit of NF-κB by western blot analysis. Phosphorylation of p65 was dose-dependently inhibited by luteolin treatment. Mitogen-activated protein kinases (MAPKs) are also important signaling molecules for COX-2 regulation. Luteolin treatment inhibited TPA-induced extracellular-signal regulated protein kinase (ERK) phosphorylation that is crucially related to inflammatory cascades regulation. These results suggest that luteolin attenuates TPA-induced COX-2 expression by blocking NF-κB activation through suppressed ERK phosphorylation in A549 cells.

목차

1. Introduction

2. Materials and Methods

3. Results and Discussion

References

키워드

luteolin,cyclooxygenase-2,NF-κB,ERK