자동 분주기 Fixed Tip의 Carryover 방지를 위한 개선 방안

• 영문명: The Prevention of Carryover in the Automated System With Fixed Tips
• 발행기관: 대한핵의학기술학회
• 저자명: 황보라(Bo Ra Hwang) 어두희(Doo Hee Au) 배진수(Jin Su Bae) 박종오(Jong O Park) 김지영(Ji Young Kim) 석재동(Jae Dong Seok)
• 간행물 정보: 『핵의학기술』Vol.13 No.2, 181~184쪽, 전체 4쪽
• 주제분류: 의약학 > 방사선과학
• 파일형태: PDF
• 발행일자: 2009.10.30

영문 초록

Purpose: Automated system that immunoassay examination are used widely. However, sample to sample carryover can cause that the next patient sample is false positive. Materials and Methods: We test HBs Ag, HBs Ab, HBc Ab(IgG) with Automated pipetting system (Tecan). It was placed with very high concentrations followed by saline solution. During this experiment, The fixed tip of Automated system wash With 0.25 moL/L NaOH. The Measurement results of saline solution confirm the occurrence of carryover. Results: Results of saline solution with 0.25 moL/L NaOH cleaning process was measured 100% negative, And results of patient serum with 0.25 moL/L NaOH cleaning process is similar reported results. Conclusion: As Results, 0.25 moL/L NaOH cleaning process was avoid carryover in experiment, And we know results of the hepatitis test did not affected by this solution . we recommend 0.25 moL/L NaOH cleaning process as the Prevention of carryover in the automated system with fixed Tips.

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키워드

TECAN Carryover Fixed Tip Automated system False positive