레시틴으로 제조된 리포좀으로부터 오계육으로부터 생산된 기능성 펩타이드의 in vitro 방출 특성

• 발행기관: 한국응용과학기술학회 (구.한국유화학회)
• 저자명: 하유진 김슬기 지중구 유선균
• 간행물 정보: 『한국유화학회 학술대회 논문집』2017년 추계학술대회, 129~129쪽, 전체 1쪽
• 주제분류: 공학 > 화학공학
• 파일형태: PDF
• 발행일자: 2017.11.02

영문 초록

Yoensan Ogae as Korean black body fowl that has been designated as a natural monument (registration number 265) is genetically distinguished from other species. The peptides such as Ala-Pro-Ala-Pro-Ala-Pro-Ala-Pro expressing the antioxidant activity produced from the meats. The bioactive peptides must retain active during digestion and absorption before entering the blood stream. Therefore, this study was to evaluate the degree of release of peptides during passing the gastrointestinal environment. To manufacture the liposoms, soy lecithin, and peptides were hydrated 10 ml distilled water containing peptides (3%, w/v). Hydration was performed for 1 h at room temperature under a nitrogen atmosphere. Then, the mixture volume was made up to 100 ml with 3% (v/v) glycerol in phosphate-buffered saline. The pH of the mixture was adjusted to 7.4 and heated to 65°C with continuous shaking for 60 min under a nitrogen atmosphere. The crude liposomes were homogenized using a high pressure homogenizer, microfluidizer, at a pressure of 17,000 psi (117.2 MPa) for 5 cycles. Chitosan-coated (CH-coated) liposomes were prepared by mixing the liposome suspensions with an equal volume of chitosan solution. In order to assess the stability and protective effects imparted by the CH coating layer, coated and uncoated liposomes were incubated in simulated-gastric fluid and simulated intestinal fluids. The peptides were analyzed by Bicinchoninic Acid (BCA) Protein Assay Kit. The Characterization of chitosan coated liposomes containing peptides were analyzed by a SEM and Zeta potential measurements. The loss of peptides were found from the both cases. CH-uncoated liposomes released peptides about 40%, but CH-coated liposomes about 20% in simulated-gastric fluid. CH-uncoated liposomes released peptides about 80%, but CH-coated liposomes about 40% in simulated-intestinal fluid.