Bioconversion of fructose to mannitol by the immobilized Leuconostoc mesenteroides sp. strain JFY on activated carbons in semi-continuous culture

• 발행기관: 한국응용과학기술학회 (구.한국유화학회)
• 저자명: Hee Jun Roh Yoo Jin Ha Do Hyun Sun Sung Ui Yoo Sun Kyun Yoo
• 간행물 정보: 『한국유화학회 학술대회 논문집』2017년 추계학술대회, 127~127쪽, 전체 1쪽
• 주제분류: 공학 > 화학공학
• 파일형태: PDF
• 발행일자: 2017.11.02

영문 초록

Mannitol: GRAS can be used anticaking, stabilizing, thickening, and texturizing as the food additives, and antioxidant for reducing plasma hydrogen peroxide as the medical treatments. Most commercial mannitol has been synthesized chemically, but strong demand for natural products caused many researchers to study the bioconversion process for mannitol production. This study reported the conversion of fructose to mannitol in a packed bed reactor in semi-continuous mode bacterial cells immobilized on the activated carbons. The strain to convert the fructose to mannitol was isolated from kimches. The immobilized reactor, a 250 x 60 mm glass column with water-cooling jacket, was packed with activated carbons (30 x 80 mesh) and connected to a peristaltic pump. Fructose (5% w/v) was pumped with an downward flow of 5 ml/min and the outlet stream was continuously recirculated into a reservoir and back to the column. Five consecutive batches using fresh medium for each one were carried out at 25℃. The highest yield was obtained at the second batch (86%) and decreased gradually (about 10%) as the batches proceeded.