흰쥐 배양 전배자 및 중뇌세포에서 Ochratoxin A의 독성

• 영문명: Embryotoxicity of Ochratoxin A in Cultured Rat Embryonic Midbrain Cells and Whole Embryos
• 발행기관: 대한약학회
• 저자명: 홍진태(Jin Tae Hong) 박귀례(Kui Lea Park) 한순영(Soon Young Han) 박기숙(Ki Sook Park) 김형식(Hyung Sik Kim) 오세동(Se Dong Oh) 박희정(Hee Jung Park) 이이다(Rhee Da Lee) 장성재(Seung Jae Jang)
• 간행물 정보: 『약학회지』제42권 제3호 (1998년), 336~344쪽, 전체 9쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1998.06.30

영문 초록

Effects of ochratoxin A (OTA) on embryo development were studied in cultured whole embryos from 9.5 day gestation rat for 48 h. OTA (more than 0.5mcg/ml) induced microcephaly in the cultured rat whole embryos. Protein and DNA content, and DNA synthesis were significantly inhibited by OTA. We next examined whether the microcephaly seen in cultured whole embryo partially results from inhibition of differentiation of embryonic midbrain cells. Embryonic midbrain cells were extracted from 12 day gestation rat embryos, and cultured for 96 hr. OTA ibhibited cell differentiation about 50% over control. We also tested whether OTA-induced embryotoxicity would be associated with oxidative damages. We measured the gamma-glutamyltranspeptidase (gamma-GT) and glutathione peroxidase (GPX) activities, and glutathione (GSH) content in both cultured whole embryos and embryonic midbrain cells. OTA decreased GSH content, whereas slightly increased gamma-GT activity, but GPX activity was not significantly changed. These results show that OTA caused the microcephaly and its effect may be partially due to the inhibition of cell differentiation of embryonic midbrain cells, but the role of oxidative damages is not clear in embryotoxicity.

키워드

Ochratoxin A microcephaly midbrain cell differentiation oxidative damages.