신규 항균물질 5-S-GAD(N-beta-alanyl-5-S-glutathionyl-3,4-dihydroxyphenylalanine)의 합성 및 생리활성

• 영문명: Synthesis and Biological Activity of 5-S-GAD(N-brta-alanyl-5-S-glutathionyl-3,4-dihydroxyphenylalanine), a Novel Antibacterial Substance
• 발행기관: 대한약학회
• 저자명: 임재윤(Jae Yoon Leem) 박호용(Ho Yong Park) Natori Shunji
• 간행물 정보: 『약학회지』제42권 제3호 (1998년), 248~256쪽, 전체 9쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1998.06.30

영문 초록

We had already reported that we purified N-beta-alanyl-5-S-glutathionyl-3,4-dihydroxyphenylalanine (5-S-GAD), a novel antibacterial substance from the immunized adult Sarcophaga peregrina (Flesh fly). We found that the antibacterial activity of synthetic 5-S-GAD is equal to that of authentic 5-S-GAD without a specificity of antibacterial activity against Gram positive and Gram negative. Significant synergism was detected between 5-S-GAD and streptomycin against streptomycin resistant strain E.coli K12 594. It has an antitumor activity against several tumor cell lines at a concentration of 100mcM. However, no cytotoxic activity against murine macrophage was detected at a concentration of 500mcM. Furthermore, haemolytic activity against sheep erythrocytes was not detected at the same concentration. We suggest that the S-conjugation of glutathion with dihydroxyphenylalanine might be important to increase antibacterial activity of dihydroxyphenylalanme.

키워드

Insect defense molecules antibacterial substance 5-S-GAD synthesis GSH synergism antitumor activity.