별불가사리 렉틴의 특성 및 암 세포 성장저해 효과

• 영문명: Characteristics and Cancerostatic Activity of the Starfish Lectin
• 발행기관: 대한약학회
• 저자명: 전경희(Kyung Hee Jeune) 박채수(Chae Soo Park) 박원학(Won Hark Park) 최수정(Soo Jeong Choi) 소명숙(Myung Suk So) 정시련(See Ryun Chung)
• 간행물 정보: 『약학회지』제41권 제4호 (1997년), 421~432쪽, 전체 12쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1997.08.30

영문 초록

A new lectin was partially purified from starfish,Asterina pectinifera by means of physiological saline extraction, salt fractionation, ion exchange chromatography and hydroxyapatite chromatography, and it was named APL. The biochemical properties of the APL were characterized. In addition, its effects on lymphocyte mitogenicity and cancer cell agglutinability were tested. The APL agglutinated nonspecifically human erythrocytes and rabbit blood cells. Agglutinability was decreased to 30% of control activity below pH 5 and above pH 9 and was relatively unstable at increasing temperatures above 60oC. The activity was reduced by addition of two kinds of metal ions, Ba2+, Mn2+ and chelating agent, EDTA. APL was proved to be glycoproteins containing 9% sugars. For carbohydrate specificity, it was found that the activity of APL was inhibited by D(+)-glucosamine, D(+)-galactosamine, stachyose, N-acetyl-galactosamine and methyl-alpha-D-galactopyranoside among 35 sugars tested. In amino acid composition, the contents of acidic amino acids such as aspartic acid and glutamic acid were relatively high. This result suggest that the isoelectric point would be in a lower range. APL was found that it promotes the division of human lymphocytes. APL was proved to be a potent agglutinin for cancer cells such as HeLa, L929 and L1210 cells. Significant changes on the HeLa cell surfaces affected by APL were observed under the electron microscope.

키워드

Asterina pectinifera lectin mitogenicity cancer cell agglutination.