고속액체크로마토그래피에 의한 벤조일 클로라이드를 유도체화제로 한 혈청 중의 폴리아민의 분리 정량

• 영문명: Determination of Polyamines in Serum Using Benzoyl Chloride as Derivatization Agent by HPLC
• 발행기관: 대한약학회
• 저자명: 이석봉(Seok Bong Lee) 고용석(Yong Seok Ko) 김형민(Hyung Min Kim) 문영회(Young Hoe Moon) 진원택(Weon Taek Jin) 신태용(Tae Yong Shin)
• 간행물 정보: 『약학회지』제41권 제4호 (1997년), 414~420쪽, 전체 7쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1997.08.30

영문 초록

A rapid and sensitive high performance liquid chromatographic method with UV detection for the analysis of polyamines(putrescine, spermidine, spermine) has been developed. The benzoyl chloride derivatives of putrescine, spermidine and spermine are separated on a mc-Bondapak C18 reverse-phase column with 50% methanol as the mobile phase. This method was applied to the analysis of polyamines in the serum of normal human and uterine cancer patients. The results show that the mean level of polyamines in cancer patients serum is much higher than that in normal human serum.

키워드

Putrescine spermidine spermine benzoyl chloride HPLC analysis.