H+ 선택성 막 전극을 이용한 바이오센서의 감응 특성

• 영문명: Characteristic Responses of Biosensor Based on H+-Selective Membrane Electrode
• 발행기관: 대한약학회
• 저자명: 김기묘(Ki Myo Kim) 박성배(Sung Bae Park) 황명석(Myoung Seok Hwang) 허문회(Moon Hye Hur) 안문규(Moon Kyu Ahn)
• 간행물 정보: 『약학회지』제40권 제6호 (1996년), 625~631쪽, 전체 7쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1996.12.31

영문 초록

The H+-selective membrane consists of tridodecylamine, 2-nitrophenyl octyl ether and a little amount of additive in carboxylated PVC matrix, where penicillinase(Pcase) and glutaraldehyde may be covalently attached to the matrix. When the H+-selective electrode was used as a detector of biosensor, calibration curve calculated from Nernst equation was not linear. So we investigated the characteristic responses of the H+-selective electrode to the product H+ from hydrolysis of penicillin-G(Pc-G) and plotted calibration curve correlating potential to concentration of Pc-G linearly. The optimal concentration of buffer solution was theoretically calculated and was also experimented. We tried to explain the linear curve of potential to concentration of Pc-G by using Henderson-Hasselbach equation. This method is more effective in calibration curve plotting than any other previous methods. The results obtained may help in further developing pH electrodes with improved analytical preformance.

키워드

H+-selective membrane Nemst equation Henderson-Hasselbalch equation.