A Simple Method for the Detection and Identification of Korean Brown Cattle(Hanwoo) using DHPLC

• 영문명: A Simple Method for the Detection and Identification of Korean Brown Cattle(Hanwoo) using DHPLC
• 발행기관: 한국축산식품학회
• 저자명: Kang, Won Park, Sane-Bum Nam, Youn-Hyoung An, Young-Chang Lee, Sang-Hyun Park, Jin-Keun Lee, J
• 간행물 정보: 『학술대회논문집』2006년도 정기총회 및 제37차 춘계 국제학술발표대회, 179~183쪽, 전체 5쪽
• 주제분류: 농수해양 > 식품과학
• 파일형태: PDF
• 발행일자: 2006.05.31

영문 초록

Denaturing high-performance liquid chromatography(DHPLC) is used in a wide variety of genetic applications and is an efficient method for detection of mutations involving one or a few nucleotides. We developed a high-throughput DHPLC method for identifying polymorphisms in the MC1R gene that are characteristic of Hanwoo cattle. We compared 10 tissue samples from Hanwoo cattle, 10 samples from Holstein cattle and 10 samples from Hanwoox Holstein crossbred cattle to determine whether DHPLC analysis can be used to distinguish between these genotypes. Samples obtained from Hanwoo cattle had a unique profile of peaks that could be used as a molecular fingerprint for this breed. We also analyzed two hundred samples in a trial in which we were blinded to the genotype of the samples and correctly identified the breed-of-origin of 594 out of 600 sequence variations(99%).

목차

Abstract

Introduction

Materials and Methods

Results and Discussion