Proteomic Analysis of Differential Protein Expression in Fibroblast-like Synoviocytes of Pig

• 영문명: Proteomic Analysis of Differential Protein Expression in Fibroblast-like Synoviocytes of Pig
• 발행기관: 한국축산식품학회
• 저자명: O, Baatartsogt. So, Hyun-Kyung Cho, In-Hee Lee, Jeong-Soo Lim, Hee-Kyung Lee, Jong-Ha Kim, Eun
• 간행물 정보: 『학술대회논문집』2006년도 정기총회 및 제37차 춘계 국제학술발표대회, 122~127쪽, 전체 6쪽
• 주제분류: 농수해양 > 식품과학
• 파일형태: PDF
• 발행일자: 2006.05.31

영문 초록

The innermost structures of synovium consist of one to three layers of cells generally identified as synovial lining cells(SLC). The present studies were initiated to determine the protein expression patterns of fibroblast-like synovial(FLS) cells derived from the synovia of rheumatoid arthritis. Post-traumatic arthritis(PTA) is one of the most common causes of secondary osteoarthritis, and usually affects younger people. The proteins were separated by two-dimensional polyacrylamide gel electrophoresis and RNA expression investigated by RT-PCR Proteome analyses led to the identification of more than 1,500 protein spots and of 11 differently expressed protein spots among them. Six proteins were down-regulated, and five proteins were up-regulated in ACL-transected synovial tissue. Among these, spots 3 and 8 were identified as cofilin-1 and smooth muscle protein 22-α, respectively, Therefore, the proteome analysis of synovial tissue is a useful approach to investigate a joint after an injury and can be used to understand the pathogenesis of PTA.

목차

Abstract

Introduction

Materials and Methods

Results

Discussion

키워드

fibroblast-like synovial cell 2-DE proteomics down-regulated up-regulated