Carboxylated PVC에 페니실리나제를 고정한 효소 센서의 특성

• 영문명: Characteristics of Enzyme Sensors using Carboxylated PVC for Immobilizing Penicillinase
• 발행기관: 대한약학회
• 저자명: 김기묘(Ki Myo Kim) 김영학(Young Hak Kim) 이은엽(Eun Yup Lee) 허문회(Moon Hye Hur) 안문규(Moon Kyu Ahn)
• 간행물 정보: 『약학회지』제40권 제1호 (1996년), 72~77쪽, 전체 6쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1996.02.28

영문 초록

Penicillin sensor was manufactured by immobillizing penicillinase with glutaraldehyde on the H+-selective membrane based on PVC-COOH-TDDA. This membrane was not interfered by K+ ion in Pc-G potassium salt. When enzyme was immobilized with glutaraldehyde, the PVC-COOH matrix was more effective than PVC matrix. Calibration curve calculated from Nernst equation was not linear. But potential was relative to concentration of Pc-G. And maximal potentiometric velocity was also relative to concentration of Pc-G. Therefore, it may be applied to Michaelis-Menten equation. The penicillin sensor was useful for determination of Pc-G at concentration of 0.1~10mM level.

키워드

Penicillin sensor penicillin PVC-COOH Michaelis- Menten equation.