일차 배양 흰쥐 간세포에서 사염화탄소 유발 세포독성에 대한 수종 생약 용매 분획의 억제효과 검색과 in vivo 간보호 작용 평가

• 영문명: Screening for Inhibitory Effect of Solvent Fractions Prepared from Herbal Drugs on CCl4-induced Cytotoxicity in Primary Cultured Rat Hepatocytes and Evaluation of Antihepatotoxicity in Vivo
• 발행기관: 대한약학회
• 저자명: 김영숙(Young Sook Kim) 경종수(Jong Su Kyung) 박기현(Ki Hyun Park)
• 간행물 정보: 『약학회지』제40권 제1호 (1996년), 52~58쪽, 전체 7쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1996.02.28

영문 초록

Solvent fractions were prepared from traditional herbal drugs which of methanol extracts inhibited CCl4-induced cytotoxicity in primary cultured rat hepatocytes and continuously assayed their effects. Ethylacetate and n-buthanol fractions from Cibotii Rhizoma and chloroform fraction from Gelatina Nigra inhibited the release of LDH and GPT from CCl4-treated hepatocytes, respectively. Water fraction (WAR) among solvent fractions from Astragali Radix showed the most potent inhibitory effect on the release of GOT or GPT by treatment with CCl4. All of solvent fractions prepared from Eucommiae Cortex had no effect on CCl4-induced cytotoxicity. Chloroform and ethylacetate fractions from Rehmanniae Radix Preparata increased the release of GPT from CCl4-treated hepatocytes. n-Hexan, chloroform or ethylacetate fraction from 5 herbal drugs increased the release of LDH, GOT or GPT from normal hepatocytes at the dose of 1.Omg/ml. Administration of WAR suppressed the elevation of GOT, ALP activities and MDA contents in the serum as well as in the liver tissue of CCl4-intoxicated rats. Based on these results, isolation of antihepatotoxic substances from WAR is under the process.

키워드

Astragali Radix antihepatotoxicity CC14 primary cultured rat hepatocytes.