광반응 HPLC를 이용한 시호 사포닌의 분석

• 영문명: Analysis of Saikosaponins by HPLC with Photoreduction Fluorescence Detection
• 발행기관: 대한약학회
• 저자명: 신영근(Young Geun Shin) 조경희(Kyung Hee Cho) 권수진(Soo Jin Kwon) 도영미(Young Mi Do) 황귀서(Gwi Seo Hwang) 박정일(Jeong Hill Park) 박만기(Man Ki Park)
• 간행물 정보: 『약학회지』제40권 제1호 (1996년), 41~45쪽, 전체 5쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1996.02.28

영문 초록

A high performance liquid chromatography using photoreduction fluorescence detection was described for the analysis of saikosaponins. Saikosaponins were separated on an NH2 column using acetonitrile and aqueous 2-tert-butylanthraquinone(t-BAQ) as mobile phase. Column effluent was passed through a 40cm PTFE capillary tube coiled around a 10W UV lamp to reduce t-BAQ to a highly fluorescent dihydroxyanthracene derivative which was detected by a fluorescence detector. The optimal concentration of t-BAQ was found to be 6X10-5M and the optimal reaction time to be 2 seconds. The detection limit for saikosaponin a and d by this method was found to be about 280ng and 80ng. The dynamic linear range was over two orders and the correlation coefficient of the calibration curve of them was 0.998.

키워드

Photoreaction HPLC photoreduction fluorescence detection 2-tert-butylanthraquinone analysis of saikosaponins Bupleurum falcatum.