Electroporation을 이용한 그람 양성 세균의 형질전환

• 영문명: Transformation of Gram-Positive Bacteria by Electroporation
• 발행기관: 대한약학회
• 저자명: 오태권(Tae Gwon Oh) 김병각(Byung Kak Kim) 최응칠(Eung Chil Choi)
• 간행물 정보: 『약학회지』제40권 제1호 (1996년), 59~64쪽, 전체 6쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1996.02.28

영문 초록

Gram-positive bacteria, Bacillus subtilis BR151 and Staphylococcus aureus RN4220 were transformed with high efficiency by electroporation. The cells were incubated until late log phase, washed three times with 10% glycerol, 1mM HEPES, 12% sucrose and resuspended to 1010~1011cfu/ml, then stored at -70oC. Transformation efficiency of B. subtilis BR151 was 1.03X107cfu/mcg with cells washed with 10% glycerol and electroporated by 15KV/cm, 0.7msec pulse with pUB110. Transformation efficiency of S. aureus RN4220 was 4X106cfu/mcg with cells washed with 1mM HEPES + 10% glycerol and electroporated by 15KV/cm, 2.5msec pulse. The number of total transformants was 1000 when B. subtilis BRI51 was transformed with 100ng pUB110 DNA and the number of total transformants was 9000 when S. aureus RN4220 was transformed with 10ng pUB110 DNA

키워드

Electroporation Bacillus subtilis BR151 Staphylococcus aureus RN4220 pUBllO.