Bifidobacterium bifidum에서 리팜피신에 대한 내성기전

• 영문명: The Mechanism of Resistance to Rifampicin in Bifidobacterium bifidum
• 발행기관: 대한약학회
• 저자명: 정영자(Young Ja Chung) 박성수(Seong Soo Park) 백문창(Moon Chang Baek) 김병각(Byong Kak Kim) 최응칠(Eung Chil Choi)
• 간행물 정보: 『약학회지』제42권 제2호 (1998년), 175~180쪽, 전체 6쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1998.04.30

영문 초록

Bifidobacterium bifidum OFR9 that exhibits acquired resistance to rifampicin and fluoroquinolones was selected by MNNG and multi-step mutation method. To investigate the resistance mechanism to rifampicin in the strain, RNA polymerase from B. bifidum parent strain and rifampicin-resistance OFR9 was partially purified and its sensitivity to rifampicin was assayed. The profile of RNA polymerase preparation of B. bifidum parent and B. bifidum OFR9 is similar to that of E. coli RNA polymerase that includes the basic subunits of beta`, beta, sigma, alpha but which are a little different in size when they are compared with E. coli RNA polymerase subunits. RNA polymerase isolated from the parent strain was inhibited by 1mcg/ml rifampicin but that from B. bifidum OFR9 was not affected by 100mcg/ml concentration of rifampicin. RNA polymerase activity of B. bifidum OFR9 was maintained over 90% through that rifampicin concentration. This result is consistent with MIC values of in vitro test. It can be concluded that the mechanism of rifampicin resistance in B. bifidum OFR9 is due to an alteration of RNA polymerase.

키워드

Bifidobacterium bifidum mutation rifampicin resistance mechanism RNA polymerase MIC.