생약으로부터 In vitro DNA 결합활성의 검색

• 영문명: Screening of In vitro DNA-binding Activity from the Crude Drugs
• 발행기관: 대한약학회
• 저자명: 김윤설(Youn Seol Kim) 정세준(Sei Joon Jeong) 신화우(Hwa Woo Shin) 김윤철(Youn Chul Kim)
• 간행물 정보: 『약학회지』제42권 제2호 (1998년), 144~148쪽, 전체 5쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1998.04.30

영문 초록

One hundred and seventeen crude drugs were screened for DNA-binding activity in vitro. The DNA-methyl green assay is a useful biochemical screen for the detection or development of biologically active compounds which bind to DNA. This assay is based upon the fact that free methyl green undergoes rapid spontaneous molecular rearrangement to its colorless carbinol base so that the liberation of the dye from DNA by displacement can be followed spectrophotometrically as a decrease in absorbance at 65Onm. Seven methanolic extracts of crude drugs including Cinnamomi Cortex spissus, Coicis Semen, Coptidis Rhizoma, Perillae Semen, Plantaginis Semen, Polygalae Radix and Zanthoxyli Fructus showed less than 2,000mcg/ml as their IC50 values. The active principles of Plantaginis Semen and Zanthoxyli Fructus were transferred into organic solvents, which showed the IC50 values with 588 and 574mcg/ml, respectively. These fractions have been selected for isolation of biologically active constituents.

키워드

DNA-binding activity DNA-methyl green assay Screening Crude drugs.