Bulbocapnine이 PC12 세포종의 도파민 함량에 미치는 영향

• 영문명: Effects of Bulbocapnine on Dopamine Content in PC12 Cells
• 발행기관: 대한약학회
• 저자명: 신정수(Jeong Soo Shin) 이명구(Myung Ku Lee)
• 간행물 정보: 『약학회지』제42권 제2호 (1998년), 170~174쪽, 전체 5쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1998.04.30

영문 초록

The effects of bulbocapnine, an aporphine isoquinoline alkaloid, on dopamine content in PC12 cells were investigated. Bulbocapnine decreased the dopamine content dose-dependently (39.2% inhibition at 2OmcM for 24 hr). The IC50 value of bulbocapnine was 22.7mcM. Dopamine content was lowered at 6 hr after exposure to bulbocapnine. And then, the decreased dopamine level was almost maintained at 36 hr and recovered to the control level at about 60 hr. Tyrosine hydroxylase, the rate limiting enzyme in the catecholamine blosynthetic pathway, was also inhibited at 20mcM of bulbocapnine by 23.1% relative to control. We, therefore, hypothesized that the inhibition of tyrosine hydroxylase by bulbocapnine with a single treatment might be partially contributed to the decrease in dopamine content in PC12 cells.

키워드

Bulbocapnine dopamine content tyrosine hydroxylase PC12 cells.