SK-HEP-1 사람 간세포에서 Protein kinase C 신호전달체계를 통한 인삼사포닌-Rg1의 DNA 합성 촉진 효과

• 영문명: Protein kinase C-mediated Stimulatory Effect of Ginsenoside-Rg1 on the Proliferation of SK-HEP-1
• 발행기관: 대한약학회
• 저자명: 공희진(Hee Jin Kong) 이광열(Kwang Youl Lee) 정은아(Eun Ah Chung) 이유희(You Hui Lee) 김신일(Shin Il Kim) 이승기(Seung Ki Lee)
• 간행물 정보: 『약학회지』제39권 제6호 (1995년), 661~665쪽, 전체 5쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1995.12.30

영문 초록

Ginsenoside-Rg1 (G-Rg1) has been shown to stimulate DNA synthetic activity in SK-HEP-1 cells. This study was therefore designed to determine in SK-HEP-1 cells whether the stimulatory effect of G-Rg1 may be mediated by protein kinase C (PKC) which is known to play a key role in the signal transduction pathway leading to the cell proliferation. Using the in situ PKC assay method, the PKC enzyme activity was determined in SK-HEP-1 cell cultures in response to G-Rg1, at 3X10-5M or phorbol 12-myristate 13-acetate (PMA) at 10-6M which increased the enzyme activity by 1.5- and 7-fold, respectively. Furthermore, G-Rg1, was also able to synergistically increase the enzyme activity by 11-fold in the cell cultures in the presence of PMA. These stimulatory effects of G-Rg1, or PMA on the DNA synthetic activity and the PKC activity were abolished by a specific PKC inhibitor, GF109203X, These results suggest that the stimulatory effect of G-Rg1 on the DNA synthetic activity may be partly due to stimulation of PKC-mediated signal transduction pathway leading to the proliferation of SK-HEP-1 cells.