자외선에 의한 면역반응의 억제를 회복시키는 면역조절물질을 생산하는 토양 Streptomyces sp.의 분리 및 동정

• 영문명: Isolation and Identification of Soil Streptomyces sp. Producing an Immunomodulator That Restores Ultraviolet B Radiation-Induced Suppression of the Immune Response
• 발행기관: 대한약학회
• 저자명: 모영근(Young Keun Mo) 신영근(Young Keun Shin) 박동진(Dong Jin Park) 김창진(Chang Jin Kim) 이종길(Chong Kil Lee) 한성순(Seong Sun Han)
• 간행물 정보: 『약학회지』제39권 제6호 (1995년), 585~592쪽, 전체 8쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1995.12.30

영문 초록

Soil microorganisms producing immunomodulators that can restore ultraviolet B (UVB) radiation-induced suppression of the immune response were screened in vitro. Exposure of freshly isolated murine epidermal cells (EC) to 180 J/m2 of UVB radiation resulted in approximately 90% impairment of accessory cell function, as measured by their ability to support anti-CD3 monoclonal antibody-induced T-cell mitogenesis. When the culture supernatants of 150 actinomycete strains were examined for their capacity to prevent or repair the UVB-induced impairment of accessory cell function, 4 of them were identified to contain immunomodulators that can restore the decreased accessory cell function. The soil isolate that showed the most effective restorative activity, G40025, was selected and further characterized. Addition of 10mcl of the culture supernatant of G4002 grown in G-media to cultures of UVB-irradiated EC right after UVB-irradiation restored the decreased accessory cell function by 58%. The immunomodulator produced by G40025 appeared to be stabled at 100oC for 10 min. Taxonomical studies by cultural, morphological, and physiological characterization showed that the soil isolate, G4002, belongs to the genus Streptomyces.