가토 위저에서 Nitric oxide에 의해 매개되는 비-아드레날린 비-콜린성 이완반응

• 영문명: Non-adrenergic and Non-cholinergic Relaxation Mediated by Nitric Oxide in the Rabbit Gastric Fundus
• 발행기관: 대한약학회
• 저자명: 홍승철(Sung Cheul Hong) 최지은(Ji Eun Choi) 한석규(Suk Kyu Han) 김영미(Young Mi Kim) 김남득(Nam Deuk Kim) 박미선(Mi Sun Park) 홍은주(Eun Ju Hong) 김진보(Jin Bo Kim)
• 간행물 정보: 『약학회지』제38권 제2호 (1994년), 149~157쪽, 전체 9쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1994.04.30

영문 초록

The role of nitric oxide(NO) as neurotransmitter in non-adrenergic non-cholinergic (NANC) relaxation induced by electrical stimulation has been studied in circular muscle strips of the rabbit gastric fundus. In the presence of atropine and guanethidine, low frequency(1-20 Hz) and short trains (5 s) of electrical stimulation induced the frequency-dependent relaxations which were not affected by adrenergic and cholinergic blockage, but abolished by tetrodotoxin, a nerve conductance blocker. L-NNA, a stereospecific inhibitor of NO biosynthesis, inhibited the relaxations induced by electrical stimulation but not affected the relaxation to exogenous NO. The effect of L-NNA was prevented by L-arginine, the precursor of the NO biosynthesis, but not by its enantiomer, D-arginine. Exogenous administration of NO(10- 100 mcM) caused the concentration-dependent relaxation which showed a similarity to those obtained with electrical stimulation. Hemoglobin, a NO scavenger, abolished the NO-induced relaxations and also markedly inhibited those evoked by electrical stimulation. Application of adenosine triphosphate(l - 10 mcM) induced concentration-independent contractions, but in high dose caused temporary contraction followed by relaxation which was not affected by L-NNA. Exogenous vasoactive intestinal polypeptide(10-100 nM) induced the concentration-dependent relaxation, while its effects were slower in onset and more persistent than those induced by short trains and low frequencies of electrical stimulation. Based on above results, it is suggested that NO is the principal neurotransmitter of NANC nerve at relaxation induced by short trains and low frequencies of electrical stimulation in the rabbit gastric fundus.

키워드

Non-adrenergic non-cholinergic (NANC) relaxation NG-nitro-L-arginine (L-NNA) hemoglobin nitric oxide vasoactive intestinal polypeptide (VIP) rabbit gastric fundus.