리포솜 지질막의 성질에 미치는 지질 조성의 영향

• 영문명: Effects of Lipid Composition on the Properties of Phospholipid Liposomal Membranes
• 발행기관: 대한약학회
• 저자명: 김민(Min Kim) 한석규(Suk Kyu Han) 김종국(Chong Kook Kim)
• 간행물 정보: 『약학회지』제38권 제2호 (1994년), 131~139쪽, 전체 9쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1994.04.30

영문 초록

Calcein-encapsulated small unilamellar vesicles of various lipid composition were prepared using the sonication technique, and their stabilities at 20oC were examined by measuring calcein leakage from the liposomes. The fluidity of these liposomal bilayers was also investigated by measuring the fluorescence polarization of DPH labelled into the liposomes. The results showed that liposomes made of PC mixtures with different acyl chain length were very stable, which may be due to the formation of interdigitated bilayer structure. The addition of cholesterol further stabilized these PC liposomes. However, addition of cholesterol reduced the encapsulation efficiences of liposomes. The fluidity of the liposomes was significantly decreased by cholesterol in the liquid crystalline state, but not changed in the gel state. These results suggest that the enhanced stability of PC mixture liposomes may be ascribed to the formation of stable interdigitated bilayer structure. In membrane-mimetic and drug-delivery studies, vesicles made of mixtures of various phospholipids are recommended instead of addition of cholesterol to the phospholipid.

키워드

Stability of liposome calcein fluorescence self-quenching method fluidity fluorescence polarization method interdigitated bilayer structure.