Xanthine oxidase 활성 및 형전환에 미치는 구리이온의 영향

• 영문명: Effect of Copper Ion on Xanthine Oxidase Activity and Type Conversion
• 발행기관: 대한약학회
• 저자명: 허근(Keun Huh) 이상일(Sang Il Lee) 박진우(Jeen Woo Park)
• 간행물 정보: 『약학회지』제38권 제2호 (1994년), 211~217쪽, 전체 7쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1994.04.30

영문 초록

Copper intoxication and disturbance of copper metabolism induced various oxygen- derived free radicals related damages. The effect of copper ion on xanthine oxidase activity and type conversion of the enzyme which is concerned to generation of reactive oxygen species, was investigated. It was observed that xanthine oxidase activity was increased by addition of copper ion in the reaction mixture in proportional to the concentration of the metal ion until 60 mcM while the enzyme activity was inhibited in higher concentration of copper treatment. On the other hand, xanthine dehydrogenase activity was inhibited by copper ion addition with concentration dependently. Preincubation of enzyme source with 30 mcM of copper ion, which concentration marked increased the xanthine oxidase activity, unchanged the enzyme activity and type conversion compare to control in vitro system. It was also observed that copper induced xanthine oxidase activity and the enzyme type conversion was protected by dithiothreitol and penicillamine. These results indicate that the increment of the type conversion of xanthine oxidase necessarilly need the presence of copper ion in enzyme assay system.

키워드

Copper ion xanthine oxidase activity type conversion.