한국인 인슈린 유전자의 클로닝 및 분석

• 영문명: Molecular Cloning and Analysis of Korean Insulin Gene
• 발행기관: 대한약학회
• 저자명: 김형민(Hyung Min Kim) 한상수(Sang Su Han) 고건일(Kun Il Ko) 손동환(Dong Hwan Son) 전창덕(Chang Duk Jun) 정헌택(Hun Taeg Chung) 김재백(Jae Baek Kim)
• 간행물 정보: 『약학회지』제37권 제5호 (1993년), 504~510쪽, 전체 7쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1993.10.30

영문 초록

Human insulin gene is consisted of the polymorphic region with the repeating units, the regulatory sequence, the structural gene including the intervening sequence, and 3''-flanking region. The polymerase chain reaction, which amplifies the target DNA between two specific primers, has been performed for the amplification of human insulin gene and simple one-step cloning of it into Escherichia coli. Out of 1727 nuceotides compared, only 4 sites were variable: 5''-regulatory region(G2101 -> AGG); IVS I(T2401 -> A); Exon II(C2411 deletion); IVS II(A2740 dejection). The variations at the G2101 and T2401 were the same as those found in one American allele. The other two variations were observed only in the specific Korean allele. And, the enzyme digestion patterns among normal, insulin dependent diabetes mellitus, and non-insulin dependent diabetes mellitus were the same. On the other hand, PCR method showed the possibility of the quickaccess for the polymorphic region in terms of the restriction fragment length of polymorphism.

키워드

Insulin insulin dependent diabetes mellitus(IDDM) non-insulin dependent diabetes mellitus(NIDDM) restriction fragment length of polymorphism (RFLP).