흰쥐의 뮤시몰투여에 의한 배뇨반사억제효과에 대한 벤조디아제핀 수용체의 영향

• 영문명: Central Involvement of Benzodiazepine Receptor on the Muscimol-induced Inhibition of Micturition Reflex in Rats
• 발행기관: 대한약학회
• 저자명: 허인회(In Hoi Huh) 오호정(Ho Jung Oh)
• 간행물 정보: 『약학회지』제36권 제5호 (1992년), 496~505쪽, 전체 10쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1992.10.29

영문 초록

The correlation between GABA receptors(GABAA and GABAB receptor) and benzodiazepine receptor on the saline infusion-induced micturition reflex contraction was studied in the female rat. To investigate the effect of gammar-aminobutyric acid(GABA) on the micturition reflex, exogenous GABA(10mg/kg) and GABA transaminase inhibitor(aminooxyacetic acid; AOAA 1mcg) were administered intravenously(i.v.) and intracerebroventriculary(i.c.v.), respectively. In result, both GABA and AOAA inhibited the saline induced micturition reflex contraction. This AOAA induced inhibition of micturition reflex was blocked by both bicuculine, GABAA receptor antagonist, and Ro 15-1788, benzodiazepine receptor antagonist. Muscimol, GABAA receptor antagonist(1mcg i.c.v., 3mcg intrathecal; i.t., 1mg/kg i.v.) and baclofen, GABAB receptor agonist(0.1mcgi.c.v., 3mcg i.t., 1mg/kg i.v.) also inhibited the bladder contraction. Pretreatment of bicuculline(1mcg i.c.v.), but not of 5-aminovaleric acid(AVA, 1mcg i.c.v.), GABAB receptor antagonist blocked the central inhibition of muscimol. These inhibitory effects were reversed by Rol5-1788 but were potentiated by flurazepam, benzodiazepine receptor antagonist. On the other hand, the inhibitory effects of baclofen were not affected by Ro 15-1788. Diazepam and flurazepam also inhibited the micturition reflex contraction when they were administered 3mcg i.c.v., 10mcg i.t., 10mcM, 30mcM transurethrally, respectively. In conclusion, these results suggest that the micturition reflex is mediated by GABAA, GABAB receptor and benzodiazepine receptor. The bezodiazepines increase the receptor binding of GABA to the GABAA receptor, so that the benzodiiazepines show the synergistic effect on the inhibition of the micturition reflex contraction, but not to the GABAB receptor.