고속액체크로마토그라피에 의한 프로스타글란딘류의 고감도 형광 분석법

• 영문명: A Sensitive Fluorescent Detection Method for Prostaglandins by High Performance Liquid Chromatography
• 발행기관: 대한약학회
• 저자명: 이용문(Yong Moon Lee) 문동철(Dong Cheul Moon)
• 간행물 정보: 『약학회지』제36권 제5호 (1992년), 506~511쪽, 전체 6쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1992.10.29

영문 초록

The Prostaglandins were derivatized rapidly with monodansyl cadaverine as a fluorophore in mild conditions. The carboxylic moiety of prostaglandins was activated with diethyl phosphorocyanidate and successively coupled with fluorophore in dimethylformamide at room temperature. The labeling yield was reached about 95% at 15 min using arachidic acid (C20 : 0) as a test sample. This derivative showed constant fluorescent intensity at 4oC for 180 days. The derivatives of prostaglandins were shown high solvent selectivity with tetrahydrofuran in reversed-phase column. therefore, these derivatives could be successfully separated on YMC pack A-212(S-5 120A C8) column in tetrahydrofuran-based eluents. The detection limits of these derivatives was ca. 500 fmol and determination limits was ca. 5 pmol as injected amount in fluorescent detection (lambdaex. 340nm, lambaem. 520nm). In this method, the ranges of recovery and coefficient of variation were 93.6~102.7% and 4.3~5.8%, respectively.