재조합 효모균이 생산하는 Peroxidase(PA1)의 효소특성 연구

• 영문명: Characterization of Peroxidase(PA1) Produced by Recombinant Saccharomyces Cerevisiae
• 발행기관: 한국산업기술융합학회(구. 산업기술교육훈련학회)
• 저자명: 김태효(Tae-Hyeo Kim)
• 간행물 정보: 『산업기술연구논문지』산업기술교육훈련논문지 제21권 4호, 97~104쪽, 전체 8쪽
• 주제분류: 공학 > 공학일반
• 파일형태: PDF
• 발행일자: 2016.12.30

영문 초록

The study on enzymatic characterization of peroxidase(POD) produced by recombinant S. cerevisiae GPD-POD1 was carried out. Purification procedures included ammonium sulfate precipitation and DEAE-cellulose column chromatography. Final POD activity and specific activity were 85.0 U/mL and 134.7 U/mg protein. The molecular weight of recombinant POD was estimated to be 53 kDa by SDS-PAGE and native-PAGE. The optimal pH and temperature for the POD activity were found to be pH 8 and 30℃, respectively. The enzymatic properties of the recombinanr POD were examined on isoelectric point, glycoprotein, metal ions and substrate specifiities.

목차

Ⅰ. 서 론

Ⅱ. 재료 및 방법

Ⅲ. 결과 및 고찰

Ⅳ. 결 론

키워드

Peroxidase(과산화효소) Recombinant(재조합) Yeast(효모) Purification(정제) Specific Activity(비활성도)