Streptomyces sp. L77-140 이 생산하는 항생물질의 분리정제 및 동정

• 영문명: Purification and Identification of an Antibiotic Produced from Streptomyces sp. L77-140
• 발행기관: 한국산업기술융합학회(구. 산업기술교육훈련학회)
• 저자명: 장흥배(Hung-Bae Chang)
• 간행물 정보: 『산업기술연구논문지』산업기술교육훈련논문지 제16권 4호, 69~75쪽, 전체 7쪽
• 주제분류: 공학 > 공학일반
• 파일형태: PDF
• 발행일자: 2011.12.30

영문 초록

In the course of the screening for microorganism producing antibiotics, Streptomyces sp. L77-140 was discovered which produced a water-soluble basic antibiotic L77-140. By using paper disk anti-microbial assay, the productivity of antibiotic from Streptomyces sp. L77-140 was tested by several carbon and nitrogen sources such as soluble starch and soybean flour, respectively. L77-140 showed anti-microbial activities against Gram-positive and Gram-negative bacteria. From the culture supernatant, L77-140 was purified by cation exchange chromatography and silicagel column chromatography. In the pH stability test, L77-140 was stable under acidic and alkali conditions. Based on the spectroscopic measurement including Ultra Violet(UV), In Frared(IR), Nuclear Magnetic Resonance (NMR) and mass spectrum(Mass) analyses, L77-140 was identified as spenolimycin.

목차

Ⅰ. 서 론

Ⅱ. 실험 방법

Ⅲ. 실험결과 및 고찰

Ⅳ. 결 론

키워드

Antibiotic(항생제) Productivity(생산성) Cation Exchange(양이온 교환) Spenolimycin(스페노리마이신) Nuclear Magnetic Resonance(핵자기공명)