HPLC-ECD를 이용한 흰쥐의 뇌 중 감마 아미노부티르산 및 글루탐산의 정량

• 영문명: Determination of gamma-Aminobutyric Acid and Glutamic Acid in Rat Brain by High Performance Liquid Chromatography with Electrochemical Detection
• 발행기관: 대한약학회
• 저자명: 강종성(Jong Seong Kang) 이순철(Soon Chul Lee)
• 간행물 정보: 『약학회지』제43권 제3호 (1999년), 300~305쪽, 전체 6쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1999.06.28

영문 초록

A sensitive and efficient assay method was applied to determine the level of glutamic acid (GA) and gamma-aminobutyric acid (GABA) in frontal cortex and hippocampus of rat administrated with ethanol and drugs. The compounds were derivatized with 0-phthalaldehyde (OPA) and 2-mercaptoethanol for precolumn analysis. The condition for the simultaneous determination of GA, GABA and beta-aminobutyric acid (BABA) by high performance liquid chromatography with electrochemical detection was reverse phase C18 column as stationary phase, 0.1M phosphate buffer containing 0.1mM Na4EDTA: methanol=55:45 (v+v) pH 3.8 as mobile phase and 0.7V electrode voltage. The stability of reaction product of GA, GABA and BABA with OPA could be increased by adding the same volume of polyethylene glycol 400 to reaction mixture. The GABA level in frontal cortex of rat was significantly decreased by the administration of picrotoxin and diazepam, but it was significantly increased by the administration of red ginseng total saponin, N-methyl-D-glucamine and (-)-deprenyl.

키워드

gamma-Aminobutyric acid glutamic acid polyethylene glycol HPLC-ECD rat brain.