Genistein이 T lymphocyte의 Apoptosis및 복강 Macrophage의 탐식능에 미치는 영향

• 영문명: Effect of Genistein on Apoptosis of T lymphocyte and Phagocytosis of Peritoneal Macrophage
• 발행기관: 대한약학회
• 저자명: 조선경(Sun Kyung Cho) 이택렬(Taek Yul Lee) 김대근(Dae Keun Kim) 오찬호(Chan Ho Oh) 소준노(June No So) 은재순(Jae Soon Eun)
• 간행물 정보: 『약학회지』제46권 제1호 (2002년), 69~74쪽, 전체 6쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2002.02.28

영문 초록

The effects of genistein on murine thymocytes for inducing apoptotic cell death and phagocytic activity of peritoneal macrophage were studied in vivo. Addition of genistein (10 and 50μM) to cultured thymocytes from BALB/C mice definitely promoted DNA fragmentation. Also, cytofluorometric analysis of these cells demonstrated a reduction in misochondrial transmembrane potential (△Ym). But, repeated administration of genistein (1 mg/mouse/day) to mice for 7 days did not cause any detectable DNA fragmentation. Genistein decreased lucigenin chemiluminescence and engu1fment of fluorescein-conjugated E.coli particles in peritoneal macrophage. These results suggest that genistein induce an apoptosis of thymocyte via reduction in △Ym and decrease phagocytic activity of peritoneal macrophage in vitro.

키워드

genistein thymocyte DNA fragmentation mitochondrial transmembrane potential macrophage phagocytosis.