Jurkat T 임파구의 세포주기 기전에 미치는 저근백피 ( Ailanthus altissima)의 효과

• 영문명: Ehect of Ailanthus altissima Water Extract on Cell Cycle Control Genes in Jurkat T Lymphocytes
• 발행기관: 대한약학회
• 저자명: 황상구(Sang Gu Hwang) 이형철(Hyung Chul Lee) 김춘관(Chun Kwan Kim) 김대근(Dae Geun Kim) 이기옥(Gi Oug Lee) 윤용갑(Young Gab Yun) 전병훈(Byung Hun Jeon)
• 간행물 정보: 『약학회지』제46권 제1호 (2002년), 18~23쪽, 전체 6쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2002.02.28

영문 초록

Ailanthus altissima has been used to settle an upset stomach, to alleviate a fever and as an insecticide. We reported that the water extract of A. altissima induced apoptotic cell death in Jurkat T.acute Iymphoblastic leukemia cells. Herel we showed the dose-dependent inhibitions of cell viability by the extract, as measured by cell morphology The cell cycle contro1 genes are considered to play important roles in tumorigenesis. The purpose of the present study is also to investigale the effect ot A. altissima on cell cycle progression and its molecular mechanism in the cells. The level of p21-proleln was Increased after treatment ot the extract, whereas both Bcl-2 and Bax protein levels were not changed. These resu1ts suggest that A. altissima Induces apoptotic cel1 death via p2l- dependent signaling pathway in furkat cells which oelete wlld type p53 checkpoint related gene products tested (cyclin D3, cyclin dependent kinase 4, retinoblastoma E2F1) were decreased In their. Protein levels in a dose-dependent manner after treatment of the extract. Taken together, these resu1ts inulcate that the increase of apoptotlc cell death by A. altissima may be due to the inhibition of cell cycle in Jurkat cells.

키워드

Ailanthus altissima water extract apoptosis cell cycle G1 checkpoint Jurkat T-ALL cells.