화장품 원료의 피부자극성과 세포독성의 관련성

• 영문명: Correlation Between Skin Irritation and Cytotoxicity of Anti-wrinkle Agents
• 발행기관: 대한약학회
• 저자명: 이은희(Eun Hee Lee) 이종권(Jong Kwon Lee) 김용규(Yong Kyu Kim) 박기숙(Ki Sook Park) 안광수(Kwang Soo Ahn) 정경미(Kyoung Mi Jung) 정해관(Jung Hai Kwan) 이선희(Sun Hee Lee) 정수연(Soo Youn Chung) 홍진태(Jin Tae Hong)
• 간행물 정보: 『약학회지』제45권 제3호 (2001년), 310~319쪽, 전체 10쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2001.06.30

영문 초록

To compare skin irritation and cytotoxicity of anti-wrinkle agents, we examined skin irritation of six anti-wrinkle agents (ascorbic acid, glycolic acid, all trans-retinoic acid, ginseng extract, retinol, EB) in New Zeland white rabbit. Cytotoxicity of these agents was determined by MTT [tetrasolium salt 3-(4,5-dimethylthiazol-2-yl)- 2,5-diphenyl tetrazolium bromide] at multi-time points in cultured HaCaT cell, a human immortalized keratinocyte cell. We then analyzed correlation between skin irritation and cytotoxicity by spearman's rank correlation analysis. All trans- retinoic acid shewed the highest primary irritation index (0.92) in skin irritation test. Being all the six agents not irritant, retinol showed the most cytotoxic agents. The correlation between skin irritation and cytotoxicity IC50 at different time point was 0.814,0.757,0.814 and 0.7 at 3,24, 48 and 72h, respectively. We also found that IC20 and IC80 of these agents showed similar correlation with skin irritation. These results therefore demonstrated that there is close correlation between skin irritation and cytotoxicity IC50 value by MTT in HaCaT cell at early time points by anti-wrinkle agents or IC20value. IC50 at earily time point or IC20 values may be reliable alter- native determinant of skin irritation.

키워드

Skin irritation anti-wrinkle agents cytotoxicity correlationship.