세균의 Methienyl-tRNA Synthetase를 저해하는 새로운 항생물질의 스크리닝

• 영문명: Screening of New Antibiotics Inhibiting Bacterial Methlonyl-tRNA Synthetase
• 발행기관: 대한약학회
• 저자명: 곽진환(Jin Hwan Kwak) 조영준(Young Jun Cho) 송난규(Nan Kyu Song)
• 간행물 정보: 『약학회지』제45권 제3호 (2001년), 245~250쪽, 전체 6쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2001.06.30

영문 초록

Aminoacyl Trna synthetase of bacteria are known as potential targets for new anti- microbial agents. To isolate new inhibitors of bacterial methionyl-Trna synthetases from natural sources, a new target-oriented screening system using whole cells which are over-expressing a target enryme was developed. Approximately 8,000 culture broths of microorganisms from soils were teated by this screening system. Among them, ten culture broths was found to contain inhibitory activity against methionyl-Trna synthetases of Escherichia coli. For the validation of the screening system, this new method was compared with in vitro enzymatic method. Seven out of 10 culture broths showed inhibitory activity against methionyl-Trna synthetases of E. coli This result showed that the new screening system was comparable to the enzyme assay. Thus we believe that our screening system as a new method can be applied for the soreening of new antibiotics inhibiting bacterial methionyl-tRNA synthetases from natural products.

키워드

Aminoacyl tRNA synthetase target-oriented screening system enzyme inhibitor antibiotics