Wheat Germ Agglutinin-conjugated Praecoxin A의 제작 및 특성

• 영문명: Preparation and Characterization of Wheat Germ Agglutinin-conjugated Praecoxln A
• 발행기관: 대한약학회
• 저자명: 김완수(Wan Soo Kim) 김만석(Man Suk Kim) 김범수(Bum Soo Kim) 이민원(Min Won Lee) 이도익(Do Ik Lee) 최영욱(Yong Wook Choi) 김은주(Eun Joo Kim) 김하형(Ha Hyung Kim)
• 간행물 정보: 『약학회지』제45권 제3호 (2001년), 302~309쪽, 전체 8쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2001.06.30

영문 초록

Wheat germ agglutinin (WGA) lectin, which binds to human melanoma cell line, was con- jugated with Praecoxin A using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide as a cross-linking agent. Physical mixture(PM) of WGA and Praecoxin A was also prepared by using a non-specific binding property of Praecoxin A to WGA. The WGA : Praecoxin A ratio in the conjugate and PM was approximately 1:18 and 1:20, respectively. The results of hemagglutination assay and enzyme-linked lectin assay indicated that the conjugate and PM maintained the lectin-like properties of the WGA. The binding ratio of coniugate was about 70% during 4-24 hr but the most of Praecoxin A was released within 24 hr in the carte of PM. These results lead to the conclusion that the conjugate is potentially useful for the formulation of injection that requires targeting for melanoma as well as sustained release at the site.

키워드

WGA lectin praecoxin A conjugate melanoma.