Anti-IL-1beta 단일클론 항체를 이용해서 발열환자의 뇨중 IL-1beta inhibitor의 확인

• 영문명: Identification of the IL-1beta inhibitor in the febrile patient urine by anti-IL-1beta monoclonal antibody
• 발행기관: 대한약학회
• 저자명: 남경수(Kyung Soo Nam) 배윤수(Yoon Soo Bae) 남명수(Myoung Soo Nam) 오은숙(Eun Sook Oh) 박순희(Soon Hee Park) 최인성(In Seong Choe) 정태화(Tai Wha Chung)
• 간행물 정보: 『약학회지』제37권 제4호 (1993년), 420~426쪽, 전체 7쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1993.08.30

영문 초록

To effectively purify of IL-1 inhibitor from human febrile urine, we have established monoclonal antibody that reacts with human recombinant interleukin 1beta(IL-1beta). The antibody, designated ON-1, was highly specific to IL-1beta and no cross-reaction with other cytokines(IL-1alpha and IL-4) was observed. As the results of ELISA inhibition assay and Western blotting method, it was further identified that ON-1 had high binding specificity with IL-1beta. IL-1 receptor binding material from febrile patient urine was effectively purified with affinitv column chromatography which conjugated with ON-1. This urinary material inhibited the thymocyte proliferation in a dose-dependent manner. IL-1beta induced thymocyte proliferation activity was inhibited to 67.3% at 6mcg of the purified urinary material. The result may suggest that this urinary material the purified urinary material. The result may suggest that this urinary material will have antagonic effect on IL-1 action mechanism and act IL-1beta inhibitor.