Monodansyl Cadaverine유도체화된 유리지방산류의 H2O2-Bis(2,4,6-trichlorophenyl) Oxalate에 의한 고속액체크로마토그라피

• 영문명: H2O2-Bis(2,4,6-trichlorophenyl) Oxalate Chemiluminescence Detection of Monodansyl Cadaverine Derivatives of Free Fatty Acids in High Performance Liquid Chromatography
• 발행기관: 대한약학회
• 저자명: 이용문(Yong Moon Lee) 문동철(Dong Cheul Moon)
• 간행물 정보: 『약학회지』제37권 제4호 (1993년), 362~364쪽, 전체 3쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1993.08.30

영문 초록

The sensitive detection of free fatty acids was investigated by using H2O2-bis(2,4,6-trichlorophenyl) oxalate chemiluminescence system after monodansyl cadaverine labeling. Because dansyl moiety is well excited by this chemiluminescence system, monodansyl cadaverine was a prominent reagent to this system for the determination of free fatty acids. The eluent of 50mM tris-HCl buffer (pH 7.7)-acetonitrile (1 : 4, v/v) was run through TSK gel ODS 80 TM column. The reagent solutions were mixed with the eluent containing the monodansyl cadaverine derivative of fatty acids from the column. By this system, linolic acid was detected 50fmol by injected amount.

키워드

Free fatty acids monodansyl cadaverine chemiluminescence detection high-performance liquid chromatography.