Transforming growth factor β1이 배양랫트 신경교세포의 성장 및 생화학적 변화에 미치는 영향

• 영문명: Effects of TGF β₁ on the Growth and Biochemical Changes in Cultured Rat Glial Cells
• 발행기관: 대한약리학회
• 저자명: 김용식(Yong Sik Kim) 윤용하(Yong Ha Youn) 박난향(Nan Hyang Park) 박찬웅(Chan Woong Park)
• 간행물 정보: 『대한약리학잡지』제30권 제2호, 167~180쪽, 전체 14쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1994.09.30

영문 초록

Recent evidence indicates that glial cells have a wide range of funtions which are critical for maintaining a balanced homeostatic environment in the central nervous system(CNS) peripheral nervous system(PNS). Morever, astrocytes are known to participate in the tissue repair and neuroimmunologic events within the CNS through many kinds of growth factors and cytokines. We investigated the effect of TGF β₁, on the growth and biochemical changes of rat glial cells in culture. The proliferative effect was determined by ³H-thymidine uptake and the double immunostain with anti-cell-specific marker and anti-Bromodeoxyuridine(BrdU) antibody. To check the effect of biochemical changes we compared the amounts of glial fibrillar acidic protein(GFAP) and the activity of glutamine synthetase(GS) in astrocyte. And the amounts of myelin basic protein and the activity of 2 ,3 -cyclic nucleotide phosphohydrolase(CNPase) were measured in oligodendrocyte and the amounts of peripheral myelin in Schwann cell. When TGF β₁, was treated for 2 days with cultured glial cell, TGF β₁, decreased the ³H-thymidine uptake and proliferation index of double immunostain of astrocytes, which indicates the inhibition of astroglial DNA synthesis, but stimulated the growth of Schwann cell. Also, TGF β₁, decrease the GS activity and increased the amounts of GFAP in astrocyte. In the case of Schwann cells the amounts of peripheral myelin was increased when treated with TGF β₁. However, TGF β₁, didn t show any effect on the proliferation and biochemical changes in oligodendrocyte. These results suggest that TGF β₁, might have a critical action in the regulation of proliferation and biochemical changes in glial cells, especially astrocyte.

키워드

신경교세포 성장 TGF β₁ GFAP