Regeneration and Acclimatization of Regenerants in Long-term in vitro Culture of Japanese Blood Grass (Imperata cylindrica ‘Rubra’)

• 영문명: Regeneration and Acclimatization of Regenerants in Long-term in vitro Culture of Japanese Blood Grass (Imperata cylindrica ‘Rubra’)
• 발행기관: 한국자원식물학회
• 저자명: 김언약(Eon-Yak Kim) 강인진(In-Jin Kang) 이예진(Ye-Jin Lee) 양바울(Baul Yang) Vipada Kantayos 배창휴(Chang-Hyu Bae)
• 간행물 정보: 『한국자원식물학회지』제36권 제6호, 588~596쪽, 전체 9쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2023.12.31

영문 초록

Long-term culture of cell lines is an important issue in in vitro culture and in plant science. In this study, the regeneration ability and ex vitro acclimatization of regenerants were evaluated. The ploidy level of regenerants derived from long-term cultured cell lines was measured in Imperata cylindrica ‘Rubra’, Poaceae. Adventitious buds (shoots) were successfully induced from five-year-cultured calli on MS medium containing 0.1 mg/L BA or 2.0 mg/L TDZ, combined with 0.01 mg/L auxins (IAA, IBA, NAA and 2,4-D), respectively. Adventitious roots were also induced on MS medium containing 0.01 mg/L auxins (IBA, NAA and 2,4-D), respectively. Interestingly, regenerants with both red and green leaf were successfully obtained when regenerants were cultured on MS medium with 9% sucrose. Regenerants derived from long-term cultured calli were transferred to pots using an optimal acclimatization process and successfully adapted to both pot and soil conditions. Moreover, the ploidy level was measured using calli and regenerants that had been kept on MS medium containing various kinds of plant growth regulators (PGRs).

목차

Introduction

Materials and Methods

Results and Discussion

Acknowledgement

Conflicts of Interest

References

키워드

Flow cytometry In vitro organogenesis Long-term culture Plant growth regulators (PGRs) Sucrose concentration