Cryopreservation of in vitro Grown Shoot Tips of Two Freesia hybrida Cultivars by Droplet-vitrification

• 영문명: Cryopreservation of in vitro Grown Shoot Tips of Two Freesia hybrida Cultivars by Droplet-vitrification
• 발행기관: 한국자원식물학회
• 저자명: 배진주(Jinjoo Bae) 송재영(Jae-Young Song) 한지원(Ji-Won Han) 고호철(Ho Cheol Ko) 남성희(Sung-Hee Nam) 이정로(Jung-Ro Lee) 이호선(Ho-Sun Lee)
• 간행물 정보: 『한국자원식물학회지』제36권 제6호, 562~570쪽, 전체 9쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2023.12.31

영문 초록

The droplet-vitrification technique for cryopreservation has proven successful across a diverse range of germ-plasm, ensuring safe and effective long term preservation. In this study, we investigate an effective cryopreservation protocol using the droplet-vitrification technique for shoot tips of Freesia hybrida cultivars ‘Sunny Gold’ and ‘Sweet Lemon’. To determine optimal conditions for Freesia cryopreservation, we employed a carefully selected standard procedure along with additional treatments and alternative solutions. For ‘Sunny Gold’, the highest regrowth rate of 24% was achieved when shoot tips underwent dehydration with PVS3 solution for 120 minutes before direct immersion in liquid nitrogen (LN) for 1 hour, coupled with a standard protocol involving a two-step preculture with 0.3 M - 0.5 M sucrose, loading with C4 for 40 minutes, and unloading with 0.8 M sucrose for 40 minutes. In the case of ‘Sweet Lemon,’ regrowth of cryopreserved shoot tips was observed with dehydration treatments, including PVS2 (A3) for 60 minutes and PVS3 (B1) for 60 minutes, as well as longer exposure. The results reflect the distinct sensitivity of shoot tips to chemical toxicity and osmotic stress in these two genotypes. This study provides valuable evidence to consistently enhance the effectiveness of cryopreservation methods for the long-term conservation of Freesia germplasm.

목차

Introduction

Materials and Methods

Results

Discussion

Acknowledgments

Conflicts of Interest

References

키워드

Cryopreservation Droplet-vitrification Freesia Regrowth