미꾸라지 렉틴 성분의 생화학적 특성

• 영문명: Biochemical Studies on Lectins from Misgurnus spp.
• 발행기관: 대한약학회
• 저자명: 정시련(See Ryun Chung) 김장환(Jang Hwan Kim) 소명숙(Myung Suk So) 김무경(Moo Kyung Kim) 현태금(Tae Geum Hyun) 전경희(Kyung Hee Jeune)
• 간행물 정보: 『약학회지』제35권 제5호 (1991년), 444~455쪽, 전체 12쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1991.10.30

영문 초록

Two kinds of new lectin fractions (LOA-I, LOA-II) were obtained from loach (Misgurnus spp.) meat by 0.15 M NaCl extraction, salt fractionation, ion exchange and hydroxyapatite column chromatographies. On polyacrylamide gel electrophoresis, LOA-I exhibited one major and a few minor bands, but LOA-II exhibited three minor bands. The partially purified loach lectins agglutinated not only erythrocytes of human B and AB type, rabbit, dog, but also murine splenic lymphocytes. Agglutinability was relatively labile at various pH and stable at increasing temperature, but was not affected by tested several metal ions. By the sugar specificity test, D-glucosamine and methyl-beta-galactopyranose inhibited agglutinating activity at a final concentration of 3 mM. The lectins contained relatively high amounts of aspartic acid, valine and leucine, but sulfur containing amino acids, cystein, methionine and isoleucine were not determined. LOA-I, LOA-II lectins were nonmitogenic toward murine lymphocytes.

키워드

lectins hemagglutinins mitogen toxic proteins Misgurnus spp.