2-Bromoacetyltriphenylene 유도체화제를 이용한 카르복실기 함유 성분의 분석법(II) 담즙산 혼합물의 HPLC에 의한 분리정량

• 영문명: HPLC Determination of Carboxyl Group Using 2-Bromoacetyltriphenylene as a Pre-labeling Reagent Separative determination of bile acids by HPLC
• 발행기관: 대한약학회
• 저자명: 박만기(Man Ki Park) 정해수(Hai Soo Chung) 양호길(Ho Gil Yang)
• 간행물 정보: 『약학회지』제31권 제2호 (1987년), 105~111쪽, 전체 7쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1987.04.30

영문 초록

A high performance liquid chromatographic method was developed for the simultaneous determination of free and glycine conjugated bile acids. Free and glycine conjugated bile acids were extracted from bear gall bladder by methanol and from serum using a Sep-pak C18 catridge. The extracted bile acids were labeled with 2-bromoacetyltriphenylene in acetonitrile using 18-crown-6-ether as a catalyst. Derivatized bile acids were separated from the individual bile acids on a reversed-phase column (Chemcosorb 5-ODS-H) using acetonitrile-methanol-water(10:50:30) as a mobile phase and monitored by an UV-detector at 254nm. Linearities of calibration curve were obtained between 4ng and 24ng, and recoveries from bear gall bladder sample were higher than 94%.