해양 패류 렉틴성분 연구(V) 반지락조개의 렉틴성분 분리정제에 관한 연구

• 영문명: Studies on Lectins from Marine Shells(V) Isolation and Purification of Lectin from Tapes philippinarum
• 발행기관: 대한약학회
• 저자명: 정시련(See Ryun Chung) 김장환(Jang Hwan Kim) 전경희(Kyung Hee Jeune-Chung)
• 간행물 정보: 『약학회지』제31권 제2호 (1987년), 52~59쪽, 전체 8쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1987.04.30

영문 초록

The result of the screening of lectins on 28 species of marine shell fishes (mollusks) showed that 10 species (Tapes philippinarum, Cyclosunetta menstrualis, Neptunea arthritica cumingii, Omphalius pfeifferi carpenteri, Chlorostoma argyrostoma turbinatum, Chiorostoma argyrostoma lischkei, Semisulcosira corea, Neptunea polycosta, Babylonica japonica, Noverita didyma) were present hemagglutinating properties to human A, B, and 0 group, and animal blood erythrocytes. A new lectin from Tapes philippinarum was isolated and partially characterized. The lectin was purified by (NH4)2SO4 precipitation and ion exchange chromatography on DE 53 column. Six fractions were obtained from DE 53 column by salt gradient elution but only 0.3M, and 0.4M NaCl fraction had strong lectin activity. On its 0.3M NaCl fraction, purity was identified by polyacrylamide gel electrophoresis. This lectin was inhibited by N-acetyl-D-galactosamine. It seems that two kinds of lectin react as antigen by immunochemical studies.