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• 영문명: Enzyme Immunoassay for Human Chorionic Gonadotropin Using Monoclonal Antibodies
• 발행기관: 대한약학회
• 저자명: 차상훈(Sang Hoon Cha) 김학주(Hack Joo Kim) 김원배(Won Bae Kim) 양중익(Jung Ik Yang)
• 간행물 정보: 『약학회지』제31권 제2호 (1987년), 64~69쪽, 전체 6쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1987.04.30

영문 초록

Monoclonal antibodies against human chorionic gonadotropin (hCG) were prepared and characterized by examining isotype, epitope binding, cross reactivity and affinity constants. And a sandwich type enzyme immunoassay for native hCG was developed with solid phase monoclonal antibody against the conformational determinant expressed only on native hCG and horseradish peroxidase conjugated monoclonal antibody against the beta-subunit of hCG. The assay was sensitive to 1mIU hCG/ml and shown a linear response up to 200mIU hCG/ml. The cross reactivity for luteinizing hormone and beta-subunit of hCG were 1% and 0.18%, respectively.