박테리오파아지 표면 발현 시스템을 이용한 Maackia amurensis Hemagglutinin (MAH)의 기능적 발현

• 영문명: Functional Display of Maackia amurensis Hemagglutinin (MAH) on Bacteriophage
• 발행기관: 대한약학회
• 저자명: 임미정(Mi Jung Yim)
• 간행물 정보: 『약학회지』제47권 제3호 (2003년), 176~179쪽, 전체 4쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2003.06.30

영문 초록

A library of unlimited number of novel pectins with diverse specificities has been previously generated by randomly mutating the carbohydrate-recognition domain of Maackia amurensis Hemagglutinin (MAH).To establish the experimental environment capable of selecting high affinity mutant pectins in E. coli, phage display system was adapted. Carbohydrate binding capacity of two phagemid vectors, pComb3 and pComb8 displaying wild-type MAH lectin was assessed. Specific bindings of pComb3 and pComb8 phages expressing w.t. MAH to affinity-purified polyclonal anti-MAH antibody and to glycophorin was demonstrated. Both phages also showed strong hemagglutinating activity to intact but not sialidase-treated human erythrocytes, which is consistent to the specificity of native MAH. Taken togethers two different phage display vectors successfully allowed the expression of active MAH as a fusion protein on the surface of bacteriophage, which will lead to preparation of unique plant lectins with high affinity toward a variety of carbohydrate chains.

키워드

Lectin Maackia amurensis hemagglutinin(MAH) phage display system.